### R code from vignette source 'RESET_pbmc_small.Rnw' ################################################### ### code chunk number 1: RESET_pbmc_small.Rnw:16-17 ################################################### library(RESET) ################################################### ### code chunk number 2: RESET_pbmc_small.Rnw:24-32 ################################################### if (requireNamespace("Seurat", quietly=TRUE) && requireNamespace("SeuratObject", quietly=TRUE)) { SeuratObject::pbmc_small gene.names = rownames(SeuratObject::pbmc_small) gene.names[1:5] Seurat::VariableFeatures(SeuratObject::pbmc_small)[1:5] } else { message("Seurat package not available! Not executing associated vignette content.") } ################################################### ### code chunk number 3: RESET_pbmc_small.Rnw:39-54 ################################################### if (requireNamespace("Seurat", quietly=TRUE)) { gene.set.id.list = list() # Create set with top 5 variable genes gene.set.id.list[[1]] = c("PPBP", "IGLL5", "VDAC3", "CD1C", "AKR1C3") names(gene.set.id.list)[1] = "VarGenes" # Create set with 5 random genes gene.set.id.list[[2]] = c("TREML1", "CD79B", "LRRC25", "GPX1", "CFD") names(gene.set.id.list)[2] = "RandomGenes" print(gene.set.id.list) # Create the list of gene indices required by resetForSeurat() gene.set.collection = createVarSetCollection(var.names=gene.names, var.sets=gene.set.id.list) } else { message("Seurat package not available! Not executing associated vignette content.") } ################################################### ### code chunk number 4: RESET_pbmc_small.Rnw:63-71 ################################################### if (requireNamespace("Seurat", quietly=TRUE)) { pbmc.reset = resetForSeurat(seurat.data=SeuratObject::pbmc_small, num.pcs=5, gene.set.collection=gene.set.collection, k=5) } else { message("Seurat package not available! Not executing associated vignette content.") } ################################################### ### code chunk number 5: RESET_pbmc_small.Rnw:76-84 ################################################### if (requireNamespace("Seurat", quietly=TRUE)) { # Display RESET scores for first 10 cells print(pbmc.reset@assays$RESET[,1:10]) # Display overall RESET scores pbmc.reset@assays$RESET@meta.features } else { message("Seurat package not available! Not executing associated vignette content.") } ################################################### ### code chunk number 6: RESET_pbmc_small.Rnw:91-103 ################################################### if (requireNamespace("Seurat", quietly=TRUE)) { Seurat::DefaultAssay(object = pbmc.reset) = "RESET" Seurat::FeaturePlot(pbmc.reset, reduction="tsne", features="VarGenes") } else { message("Seurat package not available! Not executing associated vignette content.") oldpar = par(mar = c(0,0,0,0)) plot(c(0, 1), c(0, 1), ann = F, bty = 'n', type = 'n', xaxt = 'n', yaxt = 'n') text(x = 0.5, y = 0.5,paste("Seurat package not available!\n", "FeaturePlot not generated."), cex = 1.6, col = "black") par(oldpar) }